24 research outputs found
Development of an application for diagnosis, monitoring and rehabilitation of neurodegenerative diseases
The progress of healthcare over the last years led to an aging of the world
population, increasing the prevalence of age-related diseases in the society, mainly
neurodegenerative diseases. These pathologies currently affect more than 30
million people worldwide, and there is currently no cure for them. The high
costs experienced by patients and their families are largely due to the individual
monitoring performed by technicians. New technologies are providing alternatives
for monitoring, diagnosis and rehabilitation of individuals with these diseases. This
project includes the development of a web application which allows patients with
neurodegenerative diseases to be monitored with less invasion and costs, enabling
greater adherence. The application follows several examples from the literature for
the construction of games, includes diagnostic assessments used in the medical field
and promotes its use through gamification techniques. The construction of the web
server used the Python language and the Flask framework because of the availability
of useful modules, large supporting community and scalability. The software was
specially designed for people with Parkinson’s disease, featuring a simple navigation
between menus and an easy-to-use interface. The web application was named
"Mentalist". The development of the application was followed by professional
neurologists and the results obtained were guided by clinicians’ practice. According
to these experts, the developed functionalities cover the most important aspects of
diagnosis, monitoring and rehabilitation, making it a tool of high importance and
applicability in daily clinical practiceA melhoria das condições de vida e dos cuidados de saúde nos últimos anos tem
originado um envelhecimento da população mundial, aumentando a prevalência
de doenças relacionadas com a idade. Neste grupo de doenças destacam-se as
doenças neurodegenerativas, que afetam mais de 30 milhões de pessoas e não têm,
atualmente, cura. Os elevados custos experienciados pelos pacientes e pelas suas
famílias devem-se, em grande parte, ao acompanhamento individual realizado por
técnicos. Novas tecnologias têm possibilitado alternativas para o acompanhamento,
diagnóstico e reabilitação de indivíduos com estas doenças. Este projeto inclui o
desenvolvimento de uma aplicação web que permite a monitorização de pacientes
com menos invasão e custos, proporcionando uma maior adesão. A aplicação
segue exemplos da literatura para a construção dos jogos, inclui questionários de
diagnóstico utilizados no ramo médico e promove a sua utilização através de técnicas
de gamificação. Foi utilizada a linguagem Python e o framework Flask para a
construção do servidor devido à existência de módulos úteis, grande comunidade
de desenvolvedores e escalabilidade. O software foi desenvolvido especialmente para
pacientes com a doença de Parkinson, apresentando uma navegação simples entre
menus e uma interface fácil de usar. A aplicação web foi denominada "Mentalist".
O desenvolvimento da aplicação foi seguido por neurologistas profissionais e os
resultados obtidos foram guiados pela experiência dos clínicos. De acordo com estes
especialistas, as funcionalidades desenvolvidas contemplam os principais aspetos
relativos ao diagnóstico, monitorização e reabilitação, tornando-a num instrumento
de grande importância e aplicabilidade na prática clínica diári
description of the methodological approach
publishersversionpublishe
Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos
Submitted by sandra infurna ([email protected]) on 2016-05-24T14:46:38Z
No. of bitstreams: 1
barbara_oliveira_etal_IOC_2015.PDF: 23209973 bytes, checksum: effc89d2bc8960bf65b9af114d5e8468 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-06-02T13:08:57Z (GMT) No. of bitstreams: 1
barbara_oliveira_etal_IOC_2015.PDF: 23209973 bytes, checksum: effc89d2bc8960bf65b9af114d5e8468 (MD5)Made available in DSpace on 2016-06-02T13:08:57Z (GMT). No. of bitstreams: 1
barbara_oliveira_etal_IOC_2015.PDF: 23209973 bytes, checksum: effc89d2bc8960bf65b9af114d5e8468 (MD5)
Previous issue date: 2015Made available in DSpace on 2016-06-03T12:34:20Z (GMT). No. of bitstreams: 2
barbara_oliveira_etal_IOC_2015.PDF: 23209973 bytes, checksum: effc89d2bc8960bf65b9af114d5e8468 (MD5)
license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5)
Previous issue date: 2015Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Estado do Rio de Janeiro. UNIRIO. Rio de Janeiro, BrazilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.The yellow fever (YF) 17D vaccine is one of the most effective human vaccines ever created.
The YF vaccine has been produced since 1937 in embryonated chicken eggs inoculated
with the YF 17D virus. Yet, little information is available about the infection mechanism
of YF 17DD virus in this biological model. To better understand this mechanism, we infected
embryos of Gallus gallus domesticus and analyzed their histopathology after 72 hours of
YF infection. Some embryos showed few apoptotic bodies in infected tissues, suggesting
mild focal infection processes. Confocal and super-resolution microscopic analysis allowed
us to identify as targets of viral infection: skeletal muscle cells, cardiomyocytes, nervous
system cells, renal tubular epithelium, lung parenchyma, and fibroblasts associated with
connective tissue in the perichondrium and dermis. The virus replication was heaviest in
muscle tissues. In all of these specimens, RT-PCR methods confirmed the presence of replicative
intermediate and genomic YF RNA. This clearer characterization of cell targets in
chicken embryos paves the way for future development of a new YF vaccine based on a
new cell culture system
Kinetic Study of Yellow Fever 17DD Viral Infection in Gallus gallus domesticus Embryos
Submitted by sandra infurna ([email protected]) on 2016-06-23T19:15:34Z
No. of bitstreams: 1
pedropaulo_manso_etal_IOC_2016.PDF: 3837298 bytes, checksum: 6c380180580b1760d8c32cb01f332b2c (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-06-23T19:27:36Z (GMT) No. of bitstreams: 1
pedropaulo_manso_etal_IOC_2016.PDF: 3837298 bytes, checksum: 6c380180580b1760d8c32cb01f332b2c (MD5)Made available in DSpace on 2016-06-23T19:27:36Z (GMT). No. of bitstreams: 1
pedropaulo_manso_etal_IOC_2016.PDF: 3837298 bytes, checksum: 6c380180580b1760d8c32cb01f332b2c (MD5)
Previous issue date: 2016Submitted by Angelo Silva ([email protected]) on 2016-07-07T11:16:48Z
No. of bitstreams: 3
pedropaulo_manso_etal_IOC_2016.PDF.txt: 41790 bytes, checksum: 34550c22d039d8923094561748811b01 (MD5)
pedropaulo_manso_etal_IOC_2016.PDF: 3837298 bytes, checksum: 6c380180580b1760d8c32cb01f332b2c (MD5)
license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-07-07T12:00:32Z (GMT) No. of bitstreams: 3
license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5)
pedropaulo_manso_etal_IOC_2016.PDF: 3837298 bytes, checksum: 6c380180580b1760d8c32cb01f332b2c (MD5)
pedropaulo_manso_etal_IOC_2016.PDF.txt: 41790 bytes, checksum: 34550c22d039d8923094561748811b01 (MD5)Made available in DSpace on 2016-07-07T12:00:32Z (GMT). No. of bitstreams: 3
license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5)
pedropaulo_manso_etal_IOC_2016.PDF: 3837298 bytes, checksum: 6c380180580b1760d8c32cb01f332b2c (MD5)
pedropaulo_manso_etal_IOC_2016.PDF.txt: 41790 bytes, checksum: 34550c22d039d8923094561748811b01 (MD5)
Previous issue date: 2016Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Estado do Rio de Janeiro. UNIRIO. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Yellow fever continues to be an important epidemiological problem in Africa and South
America even though the disease can be controlled by vaccination. The vaccine has been
produced since 1937 and is based on YFV 17DD chicken embryo infection. However, little
is known about the histopathological background of virus infection and replication in this
model. Here we show by morphological and molecular methods (brightfield and confocal
microscopies, immunofluorescence, nested-PCR and sequencing) the kinetics of YFV
17DD infection in chicken embryos with 9 days of development, encompassing 24 to 96
hours post infection. Our principal findings indicate that the main cells involved in virus production
are myoblasts with a mesenchymal shape, which also are the first cells to express
virus proteins in Gallus gallus embryos at 48 hours after infection. At 72 hours post infection,
we observed an increase of infected cells in embryos. Many sites are thus affected in the
infection sequence, especially the skeletal muscle. We were also able to confirm an
increase of nervous system infection at 96 hours post infection. Our data contribute to the
comprehension of the pathogenesis of YF 17DD virus infection in Gallus gallus embryos
Total hip replacement as a solution of complications of femoral neck fractures
<p>Infected nervous tissue cells in the brain (A, B); Detail of infected cells in the brain congested with virus proteins (C,D). Yellow fever virus proteins in green, nuclei stained with DAPI in blue.</p
Nervous system of <i>Gallus gallus domesticus</i> at 72 hpi with yellow fever 17DD virus.
<p>(A) Brain section presenting some infected neurons and glial cells; (B) spinal cord infected neurons; (C) one neuron of the brain showing perinuclear thickening and vesicles dispersed throughout the cytoplasm; (D) infected fibroblastoid cells along the meninges. Yellow fever virus protein detection in green and nuclei stained with DAPI in blue.</p
Heart muscular tissue of <i>Gallus gallus domesticus</i> at 72 hpi with yellow fever 17DD virus.
<p>(A) Infected heart muscle cells; (B) desmin positive heart muscle cells showing perinuclear virus protein distribution and striated pattern compatible with sarcoplasmic virus protein distribution. Yellow fever viral antigen detection in green, nuclei stained with DAPI in blue and desmin in red.</p
Detection of viral genomic RNA in YF 17DD-infected chicken embryos.
<p>The amplicons generated by Nested-PCR were analyzed by 2% agarose gel electrophoresis. The lanes correspond to the following specimens: (1) and (2)—head; (3) and (4)—legs; (5) and (6)—wings; from (7) to (14)—trunks; (15) and (16)—vitelline membrane; (17) and (18)—chorioallantoic membrane; from (19) to (22)—negative control (water-inoculated animals). Even-numbered lanes indicate samples submitted to amplification of genomic RNA whereas odd-numbered lanes indicate samples submitted to amplification of the replicative intermediate RNA. The molecular length markers are indicated on the left of the figure. The black arrow indicates the 156bp amplicon obtained from the amplification of YF 17D RNA.</p
Confocal microscopy analysis of nervous system in <i>Gallus gallus domesticus</i> 96 hpi with Yellow Fever 17DD virus.
<p>Infected neurons in the spinal cord (A); infected cells in nerve bundles (B); infected cells in the dorsal root ganglion (C); infected fibroblastoid cells in the meninges (D). Yellow fever virus proteins in green, nuclei stained with DAPI in blue.</p
Confocal microscopy analysis of embryos of <i>Gallus gallus domesticus</i> 48 hpi with Yellow Fever 17DD virus.
<p>Mesenchymal cells in leg skeletal muscle (A) and in heart (B). Yellow fever virus in green, nuclei stained with DAPI in blue.</p